Blind fluorescence structured illumination microscopy: A new reconstruction strategy

In this communication, a fast reconstruction algorithm is proposed for fluorescence \textit{blind} structured illumination microscopy (SIM) under the sample positivity constraint. This new algorithm is by far simpler and faster than existing solutions, paving the way to 3D and/or real-time 2D reconstruction.Comment: submitted to IEEE ICIP 201

Non-invasive portable geophysical tool to monitor water content in earthen long linear infrastructures

The use of electrical conductivity measurements from a non-invasive hand held electromagnetic probe is showcased to monitor the water content of earthen embankments at routine inspections. A methodology to convert the electrical conductivity measurements from the electromagnetic device into water content values is illustrated. The methodology is based on measuring the soil electrical conductivity variation with respect to a baseline reference condition and calibrating a water content - electrical conductivity relationship by comparing electrical conductivity readings from the electromagnetic probes with water content readings taken from geotechnical probes installed in a few sections of the embankment. The values of water content converted from the conductivity measurements according to the proposed procedure were found to be in very good agreement with independent measures of water content taken at times well beyond the calibration period

Long Range Hydration Effects in Electrolytic Free Suspended Black Films

The force law within free suspended black films made of negatively charged Aerosol-OT (AOT) with added LiCl or CsCl is studied accurately using X-ray reflectivity (ca. 1{\AA}). We find an electrolyte concentration threshold above which a substantial additional repulsion is detected in the LiCl films, up to distances of 100 {\AA}. We interpret this phenomenon as an augmentation of the Debye screening length, due to the local screening of the condensed hydrophilic counterions by the primary hydration shell.Comment: 4 pages, 4 figures, to be published Phys. Rev. Let

Assessing the impact of climate change on groundwater quality of shallow coastal aquifer of eastern Dahomey basin, southwestern Nigeria

Despite the increasing interest in climate change and water security, research linking climate change and groundwater quality is still at an early stage. This study explores the seasonal effect of the change in biogeochemical process for the redox-sensitive ions and metals Fe 2+, Mn 2+, SO 4 2-, and NO 3 - to assess the groundwater quality of the shallow coastal aquifer of Eastern Dahomey Basin in southwestern Nigeria. Field physicochemical measurement of EC, pH TDS, Eh, salinity, temperature, and the static water level (SWL) was carried out on 250 shallow wells; 230 water samples were collected for analysis between June 2017 and April 2018. A spatial distribution map of these ions and metals showed an increasing concentration in the dry season water samples compared to those of the wet season. This higher concentration could be attributed to change in the intensity of hydrochemical processes such as evaporation, redox, and mineral precipitation. Results of linear regression modelling established significant relationships between SWL, SO 4 2-, NO 3 -, Fe, and Eh for both wet and dry seasons with the p-value falling between 75% and 95%, which can also be seen in the plots of Eh/ORP against Fe 2+, Mn 2+, SO 4 2-, and NO 3 -. These results revealed the influence of the redox process for both seasons, while also having a higher impact in the dry season while variation of concentration revealed decrease with increase in depth, which could be attributed to a decrease in well hydraulic properties and aeration. An Eh-pH geochemical diagram revealed NO 3 - as the controlling biogeochemical process over Fe in most of the sample wells. Concentrations of NO 3 -, Fe, and Mn are above the World Health Organization's (WHO) standard for drinking water in most water samples. This study has established the link between climate change and groundwater quality in shallow coastal aquifers and suggested the need for strategic groundwater management policy and planning to ameliorate groundwater quality deterioration

Hydrostratigraphic characterisation of shallow coastal aquifers of Eastern Dahomey Basin, S/W Nigeria using integrated hydrogeophysics; implication for saltwater intrusion

This study employed electrical resistivity tomography (ERT) in characterising the shallow groundwater aquifers of Eastern Dahomey basin in southwestern Nigeria to assess the possible occurrence and distribution of saltwater within the aquifers. Electrical resistivity tomography (ERT), induced polarization (IP) and borehole logging were carried in locations with relatively enhanced electrical conductivity (EC) within the coastal zone of the basin through 97 groundwater samples from shallow wells and boreholes; 500 m-length ERT and IP sections were carried out along three traverses A–B, C–D and E–F in directions perpendicular and parallel to the coastline. Three geoelectrical layers were identified along traverse line A–B which comprises cross-sections 1, 2, 3 and 4 located around Ugbonla, Aboto and Igbokoda with layers’ resistivity and chargeability values ranging from (1–1000, 33–200 and 1–1700 Ωm), and (−50–200 Ωm, −30–200 Ωm and −50–120 Ωm, respectively, from the top to the bottom layer. These values indicated unconsolidated sand/lateritic silty clay, underlain by a sandy/silty clay layer with underlying fine-grained sand with disseminated clay lenses. The average thickness of the first two layers was 16 and 53 m while that of the third layer was undetermined. Resistivity and chargeability results from ERT and IP cross-sections along profile C–D exhibited characteristics similar to that of profile A–B with unconsolidated sands which were underlain by intercalation of sandy/silty clay and fine-grained sands with suspected clay lenses saturated with saline water. Profile E–F revealed a geoelectrical layer with low resistivity which ranged from 1–30 Ωm with the corresponding chargeability between −150–400 ms. This indicated a saline water-saturated layer of fine-grained sand and silty clay which is overlaid by the unconsolidated unconfined freshwater aquifer. Correlation of selected ERT results with borehole logs further affirmed the suspected lithology from the sections. Two scenarios of saltwater intrusions into coastal freshwater aquifer were suggested which include the presence of trapped salt-saturated clay lenses within aquifer lithology and seawater incursion induced by over-drafting of groundwater in this basin.Therefore, it identified the need for further investigation which will involve a combination of hydrochemical and isotopes to further understand the paleowater hypothesis

An experimental investigation of the independent effect of suction and degree of saturation on very small-strain stiffness of unsaturated sand

The paper presents an experimental investigation of very small strain stiffness of unsaturated sand. A triaxial test apparatus was equipped with bender elements and compression discs in order to assess the stiffness at very small strains by measuring the velocity of propagation of shear and compression waves through an unsaturated sample. The negative water column method was adopted to apply suction at the base of the sample. The experiments were designed to investigate the independent effect of suction and degree of saturation on the wave propagation velocities. This was achieved by testing the sand sample on both the drying and wetting path

A procedure for localisation and electrophysiological characterisation of ion channels heterologously expressed in a plant context

BACKGROUND: In silico analyses based on sequence similarities with animal channels have identified a large number of plant genes likely to encode ion channels. The attempts made to characterise such putative plant channels at the functional level have most often relied on electrophysiological analyses in classical expression systems, such as Xenopus oocytes or mammalian cells. In a number of cases, these expression systems have failed so far to provide functional data and one can speculate that using a plant expression system instead of an animal one might provide a more efficient way towards functional characterisation of plant channels, and a more realistic context to investigate regulation of plant channels. RESULTS: With the aim of developing a plant expression system readily amenable to electrophysiological analyses, we optimised experimental conditions for preparation and transformation of tobacco mesophyll protoplasts and engineered expression plasmids, that were designed to allow subcellular localisation and functional characterisation of ion channels eventually in presence of their putative (possibly over-expressed) regulatory partners. Two inward K(+ )channels from the Shaker family were functionally expressed in this system: not only the compliant KAT1 but also the recalcitrant AKT1 channel, which remains electrically silent when expressed in Xenopus oocytes or in mammalian cells. CONCLUSION: The level of endogenous currents in control protoplasts seems compatible with the use of the described experimental procedures for the characterisation of plant ion channels, by studying for instance their subcellular localisation, functional properties, structure-function relationships, interacting partners and regulation, very likely in a more realistic context than the classically used animal systems

Fluorescence blind structured illumination microscopy: a new reconstruction strategy

International audienceIn this communication, a fast reconstruction algorithm is proposed for fluorescence blind structured illumination mi-croscopy (SIM) under the sample positivity constraint. This new algorithm is by far simpler and faster than existing solutions , paving the way to 3D and real-time 2D reconstruction

Two-photon fluorescence isotropic-single-objective microscopy

International audienceTwo-photon excitation provides efficient optical sectioning in three-dimensional fluorescence microscopy, independently of a confocal detection. In two-photon laser-scanning microscopy, the image resolution is governed by the volume of the excitation light spot, which is obtained by focusing the incident laser beam through the objective lens of the microscope. The light spot being strongly elongated along the optical axis, the axial resolution is much lower than the transverse one. In this Letter we show that it is possible to strongly reduce the axial size of the excitation spot by shaping the incident beam and using a mirror in place of a standard glass slide to support the sample. Provided that the contribution of sidelobes can be removed through deconvolution procedures, this approach should allow us to achieve similar axial and lateral resolution